Protein Production, Vestec near Prague, Czech Republic

About Protein Production, Vestec near Prague, Czech Republic

The core facility for protein production provides services covering DNA cloning and DNA plasmid preparation, protein expression in E. coli expression systems and recombinant protein purification. Our services can also include optimization of expression and purification protocols, protein identity and purity testing and determination of protein concentration.

Service Availability:

Remote

Physical

Instruments Available:

User Guide

Cloning services
- DNA plasmid preparation
- Cloning using traditional restriction enzymes
- Restriction-free methodology
- Mutagenesis
- Checking or preparation of the DNA templates
Recombinant protein production
- Protein expression in E. coli expression systems
- Small scale expression and solubility tests
Recombinant protein purification
- Affinity chromatography (IMAC, StrepTactinXT)
- Ion-exchange chromatography
- Size exclusion chromatography
Optimization of expression and purification protocols
Protein concentration and purity testing
Other services
- DNA isolation from maxipreps
- preparation of competent cells
- dialysis
- protein cleavage
- and more